Tcte1^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tcte1^em1Osb
• Name: t-complex-associated testis expressed 1; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6111193
• Synonyms: Tcte1^em1Maik, Tcte1^FLAG1, Tcte1^tm2(Maik)
• Gene: Tcte1 Location: Chr17:45834360-45853605 bp, + strand Genetic Position: Chr17, 22.54 cM, cytoband C
• Alliance: Tcte1^em1Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243766
• Parent Cell Line: EGR-G01 (ES Cell)
• Strain of Origin: (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Endonuclease-mediated (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Using an sgRNA and CRISPR/Cas9 technology, a FLAG sequence was inserted at the 3' end of the coding sequence immediately upstream of the stop codon. The sgRNA- and Cas9-expressing plasmid and reference plasmid were transfected into EGR-G01 ES cells. The ES cells were then injected into 8-cell ICR embryos. (J:243766)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcte1 Mutation: 29 strains or lines available

References

• Original: J:243766 Castaneda JM, et al., TCTE1 is a conserved component of the dynein regulatory complex and is required for motility and metabolism in mouse spermatozoa. Proc Natl Acad Sci U S A. 2017 Jul 03;114(27):E5370-E5378
• All: 2 reference(s)