1700012B09Rik^tm1.1Gos
Targeted Allele Detail

Summary

• Symbol: 1700012B09Rik^tm1.1Gos
• Name: RIKEN cDNA 1700012B09 gene; targeted mutation 1.1, Achim Gossler
• MGI ID: MGI:6110839
• Synonyms: 1700012B09Rik^lacZ
• Gene: 1700012B09Rik Location: Chr9:14667883-14682326 bp, - strand Genetic Position: Chr9, 4.31 cM, cytoband A3
• Alliance: 1700012B09Rik^tm1.1Gos page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:249115
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129 or CAST

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The region from the translational start codon to the start of the second intron 3 of the splice consensus site was replaced with the lacZ reporter and a frt-flanked neomycin resistance positive selection cassette by recombineering. Flp-mediated recombination removed the neo cassette. RT-PCR confirmed no products indicating transcription and splicing of exon 3 and 4 in homozygous E12.5 head, and E18.5 and adult lung tissue. However, a product in adult testis RNA at low levels indicated unusual transcription and spicing of exon 3 and 4 in this tissue. Northern blot analysis from adult testis did not detect mRNA in homozygotes but did detect the reporter transcript. (J:249115)

Expression

In Mice Carrying this Mutation:

58 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any 1700012B09Rik Mutation: 2 strains or lines available

Notes

Authors in J:249115 report using hybrid 129/cast ES cells.

References

• Original: J:249115 Stauber M, et al., 1700012B09Rik, a FOXJ1 effector gene active in ciliated tissues of the mouse but not essential for motile ciliogenesis. Dev Biol. 2017 Sep 1;429(1):186-199
• All: 1 reference(s)