Gatm^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gatm^{tm1c(KOMP)Wtsi}
• Name: glycine amidinotransferase (L-arginine:glycine amidinotransferase); targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6108890
• Synonyms: Gatm flox
• Gene: Gatm Location: Chr2:122424954-122441758 bp, - strand Genetic Position: Chr2, 60.63 cM
• Alliance: Gatm^{tm1c(KOMP)Wtsi} page
• IMPC: Gatm gene page

Mutation origin

• Mutant Cell Line: EPD0218_5_E08
• Germline Transmission: Earliest citation of germline transmission: J:251830
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 122433842 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 122434710. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:251830)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gatm Mutation: 22 strains or lines available

References

• Original: J:251830 Kazak L, et al., Genetic Depletion of Adipocyte Creatine Metabolism Inhibits Diet-Induced Thermogenesis and Drives Obesity. Cell Metab. 2017 Oct 3;26(4):660-671.e3
• All: 3 reference(s)