Bag3^tm1Chen
Targeted Allele Detail

Summary

• Symbol: Bag3^tm1Chen
• Name: BCL2-associated athanogene 3; targeted mutation 1, Ju Chen
• MGI ID: MGI:6107851
• Synonyms: Bag3^m-neo
• Gene: Bag3 Location: Chr7:128125340-128148705 bp, + strand Genetic Position: Chr7, 70.26 cM
• Alliance: Bag3^tm1Chen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:246352
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Humanized sequence) Mutations: Insertion, Single point mutation Mutation details: Codon 455 GAA encoding glutamic acid in exon 4 was changed to AAA encoding lysine (p.E460K) and an FRT site flanked neomycin selection cassette was inserted into intron 3. This mutation is equivalent to the human p.E455K mutation associated with dilated cardiomyopathy (DCM). Western blot analysis confirmed expression of the protein in the hearts comparable to BAG3 protein levels in wild-type controls. (J:246352)

Generation of the Bag3^tm1Chen allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bag3 Mutation: 29 strains or lines available

References

• Original: J:246352 Fang X, et al., Loss-of-function mutations in co-chaperone BAG3 destabilize small HSPs and cause cardiomyopathy. J Clin Invest. 2017 Aug 01;127(8):3189-3200
• All: 1 reference(s)