Bckdk^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Bckdk^{tm1c(EUCOMM)Wtsi}
• Name: branched chain ketoacid dehydrogenase kinase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6105928
• Synonyms: BDK^flox
• Gene: Bckdk Location: Chr7:127503245-127508836 bp, + strand Genetic Position: Chr7, 69.82 cM
• Alliance: Bckdk^{tm1c(EUCOMM)Wtsi} page
• IMPC: Bckdk gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241307
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 127505970 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 127509174. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, removing the lacZ and neomycin sequences. The critical exon(s) is/are thus flanked by loxP sites. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:241307)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bckdk Mutation: 46 strains or lines available

References

• Original: J:241307 Ishikawa T, et al., Muscle-specific deletion of BDK amplifies loss of myofibrillar protein during protein undernutrition. Sci Rep. 2017 Jan 04;7:39825
• All: 3 reference(s)