Prox1^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Prox1^{tm1d(EUCOMM)Wtsi}
• Name: prospero homeobox 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6102941
• Synonyms: Prox1^-
• Gene: Prox1 Location: Chr1:189850232-189902911 bp, - strand Genetic Position: Chr1, 95.85 cM
• Alliance: Prox1^{tm1d(EUCOMM)Wtsi} page
• IMPC: Prox1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:249824
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 189892597 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 189895032. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). (J:249824)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prox1 Mutation: 50 strains or lines available

References

• Original: J:249824 Martinez-Corral I, et al., Nonvenous origin of dermal lymphatic vasculature. Circ Res. 2015 May 8;116(10):1649-54
• All: 1 reference(s)