Ece1^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ece1^{tm1d(KOMP)Wtsi}
• Name: endothelin converting enzyme 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6100944
• Synonyms: Ece1^delta
• Gene: Ece1 Location: Chr4:137589548-137692540 bp, + strand Genetic Position: Chr4, 70.02 cM
• Alliance: Ece1^{tm1d(KOMP)Wtsi} page
• IMPC: Ece1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:249838
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 137662119 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 137662983. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). (J:249838)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ece1 Mutation: 140 strains or lines available

References

• Original: J:249838 Kristianto J, et al., Spontaneous recombinase activity of Cre-ERT2 in vivo. Transgenic Res. 2017 Jun;26(3):411-417
• All: 1 reference(s)