Adar^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Adar^{tm1c(EUCOMM)Wtsi}
• Name: adenosine deaminase, RNA-specific; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6100937
• Synonyms: Adar^fl
• Gene: Adar Location: Chr3:89622329-89660753 bp, + strand Genetic Position: Chr3, 39.19 cM, cytoband F2
• Alliance: Adar^{tm1c(EUCOMM)Wtsi} page
• IMPC: Adar gene page

Mutation origin

• Mutant Cell Line: EPD0087_1_D11
• Germline Transmission: Earliest citation of germline transmission: J:243912
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 89646067 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 89646817. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:243912)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adar Mutation: 83 strains or lines available

References

• Original: J:243912 Snyder EM, et al., Testicular adenosine to inosine RNA editing in the mouse is mediated by ADARB1. Biol Reprod. 2017 Jan 01;96(1):244-253
• All: 1 reference(s)