Dagla^tm1Patel
Targeted Allele Detail

Summary

• Symbol: Dagla^tm1Patel
• Name: diacylglycerol lipase, alpha; targeted mutation 1, Sachin Patel
• MGI ID: MGI:6100918
• Synonyms: DAGLalpha^-
• Gene: Dagla Location: Chr19:10222629-10282241 bp, - strand Genetic Position: Chr19, 6.55 cM
• Alliance: Dagla^tm1Patel page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222187
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector comprised of an frt-flanked neomycin selection cassette and loxP sites flanking exon 9 was inserted into position 10261203 of Chromosome 19, upstream of exon 8. Adult male and female homozygous mutant mice showed a complete lack of expression of the encoded protein as assessed by western blot analysis and reductions in enzymatic activity. (J:222187)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dagla Mutation: 62 strains or lines available

References

• Original: J:222187 Shonesy BC, et al., Genetic disruption of 2-arachidonoylglycerol synthesis reveals a key role for endocannabinoid signaling in anxiety modulation. Cell Rep. 2014 Dec 11;9(5):1644-53
• All: 3 reference(s)