Gt(ROSA)26Sor^{tm1(ERT2/I-PpoI)Pobe}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(ERT2/I-PpoI)Pobe}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Philipp Oberdoerffer
• MGI ID: MGI:6099123
• Synonyms: Gt(ROSA)26Sor^{tm1(ERT2/I-PpoI,EGFP)Pobe}, I-PpoI^STOP, Ppo^STOP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(ERT2/I-PpoI)Pobe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:249781
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: A construct comprising the following elements was inserted into the host gene: a loxP site flanked STOP cassette, improved estrogen receptor nuclear translocation domain sequence fused to the cDNA for the fungal I-PpoI homing endonuclease, followed by an IRES and the EGFP fluorescent reporter gene. This creates a conditional-ready allele that will only be expressed after cre-mediated deletion of the STOP cassette and only after application of tamoxifen will the expressed endonuclease be transported to the nucleus where it targets DNA. (J:249781)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:249781 Kim J, et al., Controlled DNA double-strand break induction in mice reveals post-damage transcriptome stability. Nucleic Acids Res. 2016 Apr 20;44(7):e64
• All: 3 reference(s)