Sec24a^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sec24a^{tm1b(KOMP)Wtsi}
• Name: SEC24 homolog A, COPII coat complex component; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6093442
• Synonyms: Sec24a^gt2
• Gene: Sec24a Location: Chr11:51583090-51649172 bp, - strand Genetic Position: Chr11, 31.28 cM
• Alliance: Sec24a^{tm1b(KOMP)Wtsi} page
• IMPC: Sec24a gene page

Mutation origin

• Mutant Cell Line: EPD0099_2_E04
• Germline Transmission: Earliest citation of germline transmission: J:198477
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 51622409 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 51623181. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). (J:198477)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sec24a Mutation: 74 strains or lines available

References

• Original: J:198477 Chen XW, et al., SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion. Elife. 2013;2:e00444
• All: 1 reference(s)