Tmem18^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tmem18^{tm1c(EUCOMM)Wtsi}
• Name: transmembrane protein 18; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5925358
• Synonyms: Tmem18^flox
• Gene: Tmem18 Location: Chr12:30634442-30641218 bp, + strand Genetic Position: Chr12, 13.0 cM
• Alliance: Tmem18^{tm1c(EUCOMM)Wtsi} page
• IMPC: Tmem18 gene page

Mutation origin

• Mutant Cell Line: EPD0790_2_C08
• Germline Transmission: Earliest citation of germline transmission: J:244315
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 30635126 of Chromosome 12 upstream of the critical exons 2 and 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 2 and 3 at position 30637396. The critical exon 2 and 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exons 2 and 3. (J:244315)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmem18 Mutation: 10 strains or lines available

References

• Original: J:244315 Larder R, et al., Obesity-associated gene TMEM18 has a role in the central control of appetite and body weight regulation. Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):9421-9426
• All: 1 reference(s)