Wactm2c(EUCOMM)Wtsi
Targeted Allele Detail
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Symbol: |
Wactm2c(EUCOMM)Wtsi |
Name: |
WW domain containing adaptor with coiled-coil; targeted mutation 2c, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:5925355 |
Synonyms: |
Wacfl |
Gene: |
Wac Location: Chr18:7868832-7929028 bp, + strand Genetic Position: Chr18, 4.62 cM
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Alliance: |
Wactm2c(EUCOMM)Wtsi page
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IMPC: |
Wac gene page |
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Mutant Cell Line: |
MEPD0997_2_D04 |
Germline Transmission: |
Earliest citation of germline transmission:
J:244930
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Parent Cell Line: |
JM8A3.N1.C2 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Conditional ready) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 7904933 of Chromosome 18 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 7905647. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s).
(J:244930)
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Original: |
J:244930 de la Rosa J, et al., A single-copy Sleeping Beauty transposon mutagenesis screen identifies new PTEN-cooperating tumor suppressor genes. Nat Genet. 2017 May;49(5):730-741 |
All: |
1 reference(s) |
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