Gnaz^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Gnaz^{tm1c(EUCOMM)Hmgu}
• Name: guanine nucleotide binding protein, alpha z subunit; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5925351
• Synonyms: Gnaz^flox
• Gene: Gnaz Location: Chr10:74803009-74852739 bp, + strand Genetic Position: Chr10, 38.48 cM
• Alliance: Gnaz^{tm1c(EUCOMM)Hmgu} page
• IMPC: Gnaz gene page

Mutation origin

• Mutant Cell Line: HEPD0557_3_H07
• Germline Transmission: Earliest citation of germline transmission: J:246053
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 74826316 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 74828256. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:246053)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gnaz Mutation: 16 strains or lines available

References

• Original: J:246053 Fenske RJ, et al., The Inhibitory G Protein alpha-Subunit, Galphaz, Promotes Type 1 Diabetes-Like Pathophysiology in NOD Mice. Endocrinology. 2017 Jun 01;158(6):1645-1658
• All: 1 reference(s)