Ttc8^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ttc8^{tm1d(KOMP)Wtsi}
• Name: tetratricopeptide repeat domain 8; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5925340
• Synonyms: Bbs8^-
• Gene: Ttc8 Location: Chr12:98886833-98949497 bp, + strand Genetic Position: Chr12, 49.88 cM
• Alliance: Ttc8^{tm1d(KOMP)Wtsi} page
• IMPC: Ttc8 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:246062
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 98909097 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 98910435. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). (J:246062)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ttc8 Mutation: 35 strains or lines available

References

• Original: J:246062 Hsu Y, et al., BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment. PLoS Genet. 2017 Oct;13(10):e1007057
• All: 6 reference(s)