Cd1d1^{tm1.1(CD1D)Cres}
Targeted Allele Detail

Summary

• Symbol: Cd1d1^{tm1.1(CD1D)Cres}
• Name: CD1d1 antigen; targeted mutation 1.1, Peter Cresswell
• MGI ID: MGI:5912410
• Synonyms: hCD1d-KI
• Gene: Cd1d1 Location: Chr3:86903141-86906748 bp, - strand Genetic Position: Chr3, 37.91 cM
• Alliance: Cd1d1^{tm1.1(CD1D)Cres} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243763
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Humanized sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The human orthologous genomic sequence was inserted into the mouse genome, replacing the endogenous locus. The human sequence, consisting of chromosome 1 nucleotides 3257291-3254243 (NCBI reference sequence NW_001838531.2), was PCR-generated from a BAC. An FRT site flanked neomycin resistance gene cassette was inserted into intron 4. The neo cassette was removed through subsequent flp-mediated recombination. Expression from this allele was confirmed by RT-PCR and the translated peptide was shown to be fully functional. (J:243763)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd1d1 Mutation: 36 strains or lines available

References

• Original: J:243763 Wen X, et al., Human CD1d knock-in mouse model demonstrates potent antitumor potential of human CD1d-restricted invariant natural killer T cells. Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):2963-8
• All: 4 reference(s)