Braf^tm1.2Brum
Targeted Allele Detail

Summary

• Symbol: Braf^tm1.2Brum
• Name: Braf transforming gene; targeted mutation 1.2, Tilman Brummer
• MGI ID: MGI:5912240
• Synonyms: Braf^AVKA
• Gene: Braf Location: Chr6:39580171-39702397 bp, - strand Genetic Position: Chr6, 18.43 cM
• Alliance: Braf^tm1.2Brum page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229687
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A construct containing a floxed minigene cassette (made of 375 bp of intron 14, mouse Braf exons 15-19, 3 polyA sites and a synthetic polyadenylation signal) a mutated version of exon 15 where the TVKS motif was changed to AVKA, and an FRT flanked neo cassette was inserted replacing the endogenous exon 15. Flp mediated recombination removed the neo cassette. Cre-mediated recombination removed the floxed minigene resulting in production of a mutant transcript. The mutations of T599 and S602 to alanines blocks phosphorylation at these residues. (J:229687)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Braf Mutation: 61 strains or lines available

References

• Original: J:229687 Kohler M, et al., Activation loop phosphorylation regulates B-Raf in vivo and transformation by B-Raf mutants. EMBO J. 2016 Jan 18;35(2):143-61
• All: 2 reference(s)