Kcnma1^tm1.1Alme
Targeted Allele Detail

Summary

• Symbol: Kcnma1^tm1.1Alme
• Name: potassium large conductance calcium-activated channel, subfamily M, alpha member 1; targeted mutation 1.1, Andrea L Meredith
• MGI ID: MGI:5911897
• Synonyms: Kcnma1^fl
• Gene: Kcnma1 Location: Chr14:23342356-24055173 bp, - strand Genetic Position: Chr14, 12.92 cM
• Alliance: Kcnma1^tm1.1Alme page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229605
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: A lox71 site was inserted upstream of exon 2 and an FRT flanked neo cassette, 2A-tdTomato gene and lox66 site in inverse orientation were inserted downstream of exon 2. Flp mediated recombination removed the neo cassette. In the absence of cre-mediated recombination the tdTomato reporter is not expressed. Following cre-mediated the tdTomato reporter is expressed and exon 2 is placed in inverse orientation. The cre-mediated inversion is irreversible. (J:229605)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kcnma1 Mutation: 105 strains or lines available

References

• Original: J:229605 Zemen BG, et al., Generation of Kcnma1fl-tdTomato, a conditional deletion of the BK channel alpha subunit in mouse. Physiol Rep. 2015 Nov;3(11)
• All: 2 reference(s)