Cd82^tm1.1Cmir
Targeted Allele Detail

Summary

• Symbol: Cd82^tm1.1Cmir
• Name: CD82 antigen; targeted mutation 1.1, Cindy Miranti
• MGI ID: MGI:5911107
• Gene: Cd82 Location: Chr2:93249447-93293295 bp, - strand Genetic Position: Chr2, 51.62 cM
• Alliance: Cd82^tm1.1Cmir page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229552
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A single loxP site was inserted 5' to exon 4 and a neomycin selection cassette followed by a second loxP site was inserted 3' to exon 5. Cre-mediated recombination resulted in the deletion of exons 4 and 5 and the selection cassette. RT-PCR analysis showed no detectable transcript in kidney, liver and spleen of homozygous mice. Immunoblotting experiments showed no detectable encoded protein in kidney, liver, spleen and prostate. (J:229552)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd82 Mutation: 16 strains or lines available

References

• Original: J:229552 Uchtmann K, et al., Homozygous loss of mouse tetraspanin CD82 enhances integrin alphaIIbbeta3 expression and clot retraction in platelets. Exp Cell Res. 2015 Dec 10;339(2):261-9
• All: 4 reference(s)