Kdm5b^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kdm5b^{tm1d(EUCOMM)Wtsi}
• Name: lysine demethylase 5B; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5910966
• Synonyms: Jarid1b^-
• Gene: Kdm5b Location: Chr1:134487916-134560621 bp, + strand Genetic Position: Chr1, 58.24 cM, cytoband E4
• Alliance: Kdm5b^{tm1d(EUCOMM)Wtsi} page
• IMPC: Kdm5b gene page

Mutation origin

• Mutant Cell Lines: EPD0027_1_F07, EPD0027_1_F08
• Germline Transmission: Earliest citation of germline transmission: J:223051
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 136491938 of Chromosome 1 upstream of the critical exon 6 (Build 37). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 6 at position 136492740. The critical exon 6 is thus flanked by loxP sites. A knock-out allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ/neomycin cassette, followed by cre recombinase to remove the floxed exon 6. (J:223051)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kdm5b Mutation: 201 strains or lines available

References

• Original: J:223051 Albert M, et al., The histone demethylase Jarid1b ensures faithful mouse development by protecting developmental genes from aberrant H3K4me3. PLoS Genet. 2013 Apr;9(4):e1003461
• All: 2 reference(s)