Cbx2^em1Rek
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cbx2^em1Rek
• Name: chromobox 2; endonuclease-mediated mutation 1, Robert E Kingston
• MGI ID: MGI:5910878
• Synonyms: Cbx2^13KRA
• Gene: Cbx2 Location: Chr11:118913845-118922101 bp, + strand Genetic Position: Chr11, 83.33 cM
• Alliance: Cbx2^em1Rek page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Hypomorph)
• Mutation: Nucleotide substitutions
• Mutation details: A portion of the part of the gene coding for the compaction region, one of the active domains of the protein, was replaced with synthetic sequence where 13 selected lysine and arginine codons had been changed to alanine codons. This was done through CRISPR/Cas9 endonuclease-assisted homologous recombination where the transgene vector, two sgRNAs and Cas9 mRNA were injected into C57BL/6 zygotes. The allele codes for a protein with redsuced activity. (J:242063)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cbx2 Mutation: 38 strains or lines available

References

• Original: J:242063 Lau MS, et al., Mutation of a nucleosome compaction region disrupts Polycomb-mediated axial patterning. Science. 2017 Mar 10;355(6329):1081-1084
• All: 2 reference(s)