Cbx2^tm1.1Rek
Targeted Allele Detail

Summary

• Symbol: Cbx2^tm1.1Rek
• Name: chromobox 2; targeted mutation 1.1, Robert E Kingston
• MGI ID: MGI:5910877
• Synonyms: Cbx2^23KRA
• Gene: Cbx2 Location: Chr11:118913845-118922101 bp, + strand Genetic Position: Chr11, 83.33 cM
• Alliance: Cbx2^tm1.1Rek page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242063
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Nucleotide substitutions
• Mutation details: A loxP site flanked neomycin resistance gene cassette was inserted. The part of the gene coding for the compaction region, one of the active domains of the protein, was replaced with synthetic sequence where 23 selected lysine and arginine codons had been changed to alanine codons. The neo cassette was removed through subsequent cre-mediated recombination. The result is an inactive protein. (J:242063)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cbx2 Mutation: 38 strains or lines available

References

• Original: J:242063 Lau MS, et al., Mutation of a nucleosome compaction region disrupts Polycomb-mediated axial patterning. Science. 2017 Mar 10;355(6329):1081-1084
• All: 2 reference(s)