Casp12^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Casp12^{tm1d(KOMP)Wtsi}
• Name: caspase 12; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5910735
• Synonyms: caspase-12^-
• Gene: Casp12 Location: Chr9:5345430-5373032 bp, + strand Genetic Position: Chr9, 2.46 cM, cytoband A1
• Alliance: Casp12^{tm1d(KOMP)Wtsi} page
• IMPC: Casp12 gene page

Mutation origin

• Mutant Cell Line: EPD0727_2_B01
• Germline Transmission: Earliest citation of germline transmission: J:235749
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 5351767 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 5353109. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). (J:235749)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Casp12 Mutation: 32 strains or lines available

References

• Original: J:235749 Vande Walle L, et al., Does caspase-12 suppress inflammasome activation?. Nature. 2016 Jun 2;534(7605):E1-4
• All: 3 reference(s)