Tg(CAG-His3.3Rainbow)1Dean
Transgene Detail

Summary

• Symbol: Tg(CAG-His3.3Rainbow)1Dean
• Name: transgene insertion 1, Jurrien Dean
• MGI ID: MGI:5910461
• Synonyms: HISRainbow
• Transgene: Tg(CAG-His3.3Rainbow)1Dean Location: unknown
• Alliance: Tg(CAG-His3.3Rainbow)1Dean page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Conditional ready, Dominant negative, Inserted expressed sequence, Reporter)
• Mutations: Insertion, Single point mutation
• Tg(CAG-His3.3Rainbow)1Dean expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  Drosophila melanogaster	His3.3A (33736)	Cre recombination induces random expression of wild-type or mutant histone His3.3A/fluorescent fusion proteins

• Mutation details: Following Cre-mediated recombination, cells of mice bearing this transgene randomly express one of three histone 3.3 fusion proteins joining different fluorescent markers to either wild-type or one of two dominant-negative mutant forms (arginine-->lysine at amino acid (aa) position 26 (R26K) or lysine-->arginine at aa 27 (K27R)) of the Drosophila melanogaster histone 3.3A protein, whose sequence is identical to the mammalian histones 3.3A and 3.3B. This is accomplished by Cre recombinase-mediated deletion of sequences between pairs of lox2272, loxN or loxP sites, one member of each pair located in a cluster between a CAG promoter and a triple poly[A] (STOP) signal and the other upstream of a cDNA encoding one of the HIS3.3A fusion proteins - HIS3.3A/EGFP, HIS3.3A*R26K/ECFP, or HIS3.3A*K27R/mCherry - followed by a poly[A] signal. A single frt site following the last poly[A] was included in the transgenic construct to allow flp recombinase-mediated deletion of all except the single remaining copy of the transgene in progeny of the multi-copy founder mouse. Expression of the fusion genes is driven by the CAG promoter, which includes the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene. (J:239167)
• Inheritance: Other (see notes)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

When expressed, both mutant histone proteins behave as dominant negative.

References

• Original: J:239167 Zhou L, et al., Genetic mosaics and time-lapse imaging identify functions of histone H3.3 residues in mouse oocytes and embryos. Development. 2017 Feb 01;144(3):519-528
• All: 1 reference(s)