Tg(Actb-EGFP,-Diaph1)#Cpm
Transgene Detail

Summary

• Symbol: Tg(Actb-EGFP,-Diaph1)#Cpm
• Name: transgene insertion, Christopher P Mack
• MGI ID: MGI:5910313
• Synonyms: DNmDia
• Transgene: Tg(Actb-EGFP,-Diaph1)#Cpm Location: unknown
• Alliance: Tg(Actb-EGFP,-Diaph1)#Cpm page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(Actb-EGFP,-Diaph1)#Cpm expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Diaph1 (MGI:1194490)	dominant negative construct lacking N-terminal half and more

• Mutation details: A vector containing the mouse beta actin promoter, a loxP site flanked EGFP gene and STOP cassette, and the cDNA for the dominant negative mutant construct of the mouse gene (F1FHdelta1) with a FLAG tag, was used to generate this allele. This creates a conditional-ready allele that will only express the mouse gene after the EGFP-STOP cassette is removed through cre-mediated recombination. No line information was given (denoted by the pound sign #). (J:242113)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:242113 Weise-Cross L, et al., Inhibition of Diaphanous Formin Signaling In Vivo Impairs Cardiovascular Development and Alters Smooth Muscle Cell Phenotype. Arterioscler Thromb Vasc Biol. 2015 Nov;35(11):2374-83
• All: 1 reference(s)