Sc5d^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Sc5d^{tm1c(EUCOMM)Hmgu}
• Name: sterol-C5-desaturase; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5909263
• Synonyms: Sc5d^fl
• Gene: Sc5d Location: Chr9:42162891-42175552 bp, - strand Genetic Position: Chr9, 23.57 cM, cytoband B
• Alliance: Sc5d^{tm1c(EUCOMM)Hmgu} page
• IMPC: Sc5d gene page

Mutation origin

• Mutant Cell Line: HEPD0601_6_E11
• Germline Transmission: Earliest citation of germline transmission: J:243633
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 42169509 of Chromosome 9 upstream of the critical exon( exon 4) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 42170467. Exon 4 is thus flanked by loxP sites. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:157065, J:243633)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sc5d Mutation: 15 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 4 reference(s)