Tg(Tcra9.1-2,Tcrb9.1-2)#Lky
Transgene Detail

Summary

• Symbol: Tg(Tcra9.1-2,Tcrb9.1-2)#Lky
• Name: transgene insertion, Richard M Locksley
• MGI ID: MGI:5909066
• Synonyms: ABLE
• Transgene: Tg(Tcra9.1-2,Tcrb9.1-2)#Lky Location: unknown
• Alliance: Tg(Tcra9.1-2,Tcrb9.1-2)#Lky page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The transgene contains the rearrangement found a CD4+ Th1 clone (9.1-2) isolated from mice immunized with soluable antigens of Leishmania major. This clones expressed Valpha8.2A-JalphaTA72 and Vbeta4-Dbeta1-Jbeta1.6. The Tcra chain was completed by adding a 9 kb fragment of Calpha containing containing 0.8 kb of upstream intron, the entire coding region, and the downstream enhancer to the 3 end of the promoter-V-J-intron construct. The Tcrb chain was completed by appending a Cbeta1 and downstream-chain enhancer construct to the 3 end of the promoter-V-D-J-intron. One of three founders was used to generate the line. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:243554)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:243554 Reiner SL, et al., Control of Leishmania major by a monoclonal alpha beta T cell repertoire. J Immunol. 1998 Jan 15;160(2):884-9
• All: 1 reference(s)