Gt(ROSA)26Sor^{tm1(Ripk3)Aob}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Ripk3)Aob}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Andrew Oberst
• MGI ID: MGI:5908548
• Synonyms: RIPK3-2xFV^fl
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Ripk3)Aob} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241350
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(Ripk3)Aob} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ripk3 (MGI:2154952)

• Mutation details: The following elements were inserted in intron 1 of the host locus: a loxP site flanked STOP cassette, the Ripk3 coding sequence with a FLAG tag fused to the 5' end and two FKBP^F36V tags to the 3' end, sequence coding for the Thosea asigna virus 2A (T2A) self-cleaving peptide sequence, and the mCherry red fluorescent marker gene. This creates a conditional-ready allele that, after the removal of the stop cassette through cre-mediated recombination, would express the FLAG- and FKBP-tagged Ripk3 peptide and the mCherry reporter. (J:241350)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1061 strains or lines available

References

• Original: J:241350 Daniels BP, et al., RIPK3 Restricts Viral Pathogenesis via Cell Death-Independent Neuroinflammation. Cell. 2017 Apr 06;169(2):301-313.e11
• All: 5 reference(s)