Postn^{tm2.1(cre/Esr1*)Jmol}
Targeted Allele Detail

Summary

• Symbol: Postn^{tm2.1(cre/Esr1*)Jmol}
• Name: periostin, osteoblast specific factor; targeted mutation 2.1, Jeffery Molkentin
• MGI ID: MGI:5908450
• Synonyms: Postn^MCM, Postn-MerCreMer
• Gene: Postn Location: Chr3:54268530-54298458 bp, + strand Genetic Position: Chr3, 25.46 cM
• Alliance: Postn^{tm2.1(cre/Esr1*)Jmol} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241168
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Postn^{tm2.1(cre/Esr1*)Jmol} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Postn (MGI:1926321)

• Mutation details: Tamoxifen-inducible cre recombinase (MerCreMer: cre recombinase fused to two modified estrogen receptor ligand binding domains at both ends) cDNA was introduced, in-frame, to the ATG start site of exon 1 via homologous recombination. The plasmid also contains a diptheria toxi A cDNA and a FRT-flanked neomycin cassette for negative and positive selection. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:241168)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Postn (mouse) Summary of all recombinase alleles driven by Postn. Alliance mouse Postn gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Postn Mutation: 52 strains or lines available

References

• Original: J:241168 Kanisicak O, et al., Genetic lineage tracing defines myofibroblast origin and function in the injured heart. Nat Commun. 2016 Jul 22;7:12260
• All: 19 reference(s)