Sirt1^tm2.1Arte
Targeted Allele Detail

Summary

• Symbol: Sirt1^tm2.1Arte
• Name: sirtuin 1; targeted mutation 2.1, TaconicArtemis
• MGI ID: MGI:5908449
• Synonyms: TEKI
• Gene: Sirt1 Location: Chr10:63154784-63174814 bp, - strand Genetic Position: Chr10, 32.57 cM
• Alliance: Sirt1^tm2.1Arte page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241351
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Two substitutions (AC>GA) were engineered to change codon 522 in exon 8 from threonine to glutamine (p.Thre522Glu). Glutamine is a phosphomimetic, simulating a phosphorylated threonine and rendering the protein constitutively active. A loxP site and an FRT site flanked selectable marker cassette were inserted into intron 7. The marker cassette was removed through subsequent flp-mediated recombination. (J:241351)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sirt1 Mutation: 83 strains or lines available

References

• Original: J:241351 Lu J, et al., The phosphorylation status of T522 modulates tissue-specific functions of SIRT1 in energy metabolism in mice. EMBO Rep. 2017 May;18(5):841-857
• All: 1 reference(s)