Syt1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Syt1^{tm1c(EUCOMM)Wtsi}
• Name: synaptotagmin I; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5908401
• Synonyms: Syt1^lox
• Gene: Syt1 Location: Chr10:108333511-108846843 bp, - strand Genetic Position: Chr10, 56.52 cM
• Alliance: Syt1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Syt1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241352
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 108477841 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 108478747. The critical exon(s) is/are thus flanked by loxP sites. This conditional-ready (floxed) allele was created by flp-mediated recombination. (J:241352)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Syt1 Mutation: 38 strains or lines available

References

• Original: J:241352 Bouhours B, et al., Synaptotagmin2 (Syt2) Drives Fast Release Redundantly with Syt1 at the Output Synapses of Parvalbumin-Expressing Inhibitory Neurons. J Neurosci. 2017 Apr 26;37(17):4604-4617
• All: 5 reference(s)