Dicer1^tm1.1Tara
Targeted Allele Detail

Summary

• Symbol: Dicer1^tm1.1Tara
• Name: dicer 1, ribonuclease type III; targeted mutation 1.1, Alexander Tarakhovsky
• MGI ID: MGI:5908165
• Gene: Dicer1 Location: Chr12:104654001-104718211 bp, - strand Genetic Position: Chr12, 54.83 cM, cytoband E-F1
• Alliance: Dicer1^tm1.1Tara page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243335
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: LoxP sites were inserted to flank exon 22, which encodes the majority of the RNase III domain. Loss of this exon would result in the generation of in-frame stop codons generated by frameshift mutations, likely to destabilize the resulting transcript. Flp mediated recombination removed an FRT-flanked neo that was included in the vector. Cre-mediated recombination removed exons 22. (J:243335)

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dicer1 Mutation: 96 strains or lines available

References

• Original: J:243335 Tang F, et al., mRNA-Seq whole-transcriptome analysis of a single cell. Nat Methods. 2009 May;6(5):377-82
• All: 1 reference(s)