Dbn1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dbn1^{tm1c(KOMP)Wtsi}
• Name: drebrin 1; drebrin 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5908152
• Synonyms: DBN^loxp
• Gene: Dbn1 Location: Chr13:55621241-55635874 bp, - strand Genetic Position: Chr13, 30.06 cM
• Alliance: Dbn1^{tm1c(KOMP)Wtsi} page
• IMPC: Dbn1 gene page

Mutation origin

• Mutant Cell Line: EPD0211_3_A05
• Germline Transmission: Earliest citation of germline transmission: J:241372
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 55628889 of Chromosome 13 upstream of exon 1 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene cassette under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 55631060. The critical exons are thus flanked by loxP sites. The lacZ and neo cassettes were removed through flp-mediated recombination, creating a conditional-ready allele with exons 1-6 floxed. (J:241372)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dbn1 Mutation: 44 strains or lines available

References

• Original: J:241372 Willmes CG, et al., Investigation of hippocampal synaptic transmission and plasticity in mice deficient in the actin-binding protein Drebrin. Sci Rep. 2017 Feb 15;7:42652
• All: 5 reference(s)