Trp53^tm1.1Dmfe
Targeted Allele Detail

Summary

• Symbol: Trp53^tm1.1Dmfe
• Name: transformation related protein 53; targeted mutation 1.1, David M Feldser
• MGI ID: MGI:5907990
• Synonyms: p53^XTR
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm1.1Dmfe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:228038
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A construct consisting of an FRT site, neo cassette and an FRT, lox2272, lox5171 flanked inverted splice acceptor site-GFP-polyA cassette was inserted in intron 1. Flp mediated recombination removed the neo cassette leaving the GFP cassette unchanged. The lox sites are arranged to allow for cre mediated inversion of the GFP cassette that is fixed after 2 successive cre mediated recombination events. Inversion of the GFP cassette traps the gene. The FRT sites are arranged to allow for Flp mediated deletion of the GFP cassette restoring gene function. (J:228038)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 249 strains or lines available

References

• Original: J:228038 Robles-Oteiza C, et al., Recombinase-based conditional and reversible gene regulation via XTR alleles. Nat Commun. 2015;6:8783
• All: 3 reference(s)