Cfap157^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Cfap157^{tm1b(EUCOMM)Hmgu}
• Name: cilia and flagella associated protein 157; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5907281
• Synonyms: Cfap157^lacZ
• Gene: Cfap157 Location: Chr2:32667425-32674417 bp, - strand Genetic Position: Chr2, 22.09 cM
• Alliance: Cfap157^{tm1b(EUCOMM)Hmgu} page
• IMPC: Cfap157 gene page

Mutation origin

• Mutant Cell Line: HEPD0601_7_G05
• Germline Transmission: Earliest citation of germline transmission: J:237484
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 32671182 of Chromosome 2 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 32671954. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and the loxP-flanked exon 2. RT-PCR, northern blot and immunoprecipitation experiments confirmed the lack of expression from this allele. (J:237484)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cfap157 Mutation: 30 strains or lines available

References

• Original: J:237484 Weidemann M, et al., CFAP157 is a murine downstream effector of FOXJ1 that is specifically required for flagellum morphogenesis and sperm motility. Development. 2016 Dec 15;143(24):4736-4748
• All: 1 reference(s)