Gucy1b1^tm1.1Brou
Targeted Allele Detail

Summary

• Symbol: Gucy1b1^tm1.1Brou
• Name: guanylate cyclase 1, soluble, beta 1; targeted mutation 1.1, Peter Brouckaert
• MGI ID: MGI:5907280
• Synonyms: apo-sGC
• Gene: Gucy1b1 Location: Chr3:81939313-81981996 bp, - strand Genetic Position: Chr3, 35.97 cM
• Alliance: Gucy1b1^tm1.1Brou page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227198
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Exon 5 was replaced with a mutant version carrying an H105F point mutation and a floxed pgk-neo cassette was inserted downstream of exon 5 via homologous recombination. Cre-mediated recombination removed the neo cassette. The replacement of the wild-type histidine with a phenylalanine interferes with haem ligation resulting in loss of NO activation. (J:227198)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gucy1b1 Mutation: 42 strains or lines available

References

• Original: J:227198 Thoonen R, et al., Cardiovascular and pharmacological implications of haem-deficient NO-unresponsive soluble guanylate cyclase knock-in mice. Nat Commun. 2015;6:8482
• All: 1 reference(s)