Hhex^tm1.1Jomb
Targeted Allele Detail

Summary

• Symbol: Hhex^tm1.1Jomb
• Name: hematopoietically expressed homeobox; targeted mutation 1.1, Joshua M Brickman
• MGI ID: MGI:5906600
• Synonyms: HRS
• Gene: Hhex Location: Chr19:37423289-37429179 bp, + strand Genetic Position: Chr19, 32.28 cM, cytoband D1
• Alliance: Hhex^tm1.1Jomb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:149792
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The following elements were inserted into exon 1 after the ATG start codon: the DsRed.T3 (enhanced T3 variant of Red Fluorescent Protein) gene, an IRES, a blasticidin resistance gene (BSD), a polyA signal and a loxP site flanked hygromycin resistance gene cassette. The hygro cassette was removed through subsequent cre-mediated recombination. Fluorescence microscopy confirms expression from this allele is similar to wild-type. (J:149792)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hhex Mutation: 15 strains or lines available

References

• Original: J:149792 Morrison GM, et al., Anterior definitive endoderm from ESCs reveals a role for FGF signaling. Cell Stem Cell. 2008 Oct 9;3(4):402-15
• All: 2 reference(s)