Hhex^tm2.1Jomb
Targeted Allele Detail

Summary

• Symbol: Hhex^tm2.1Jomb
• Name: hematopoietically expressed homeobox; targeted mutation 2.1, Joshua M Brickman
• MGI ID: MGI:5906598
• Synonyms: HBD
• Gene: Hhex Location: Chr19:37423289-37429179 bp, + strand Genetic Position: Chr19, 32.28 cM, cytoband D1
• Alliance: Hhex^tm2.1Jomb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241018
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Hypomorph, Modified regulatory region, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The starting point was allele Hhex^tm1Jomb which is constructed as follows. The following elements were inserted into exon 1 after the ATG start codon: the DsRed.T3 (enhanced T3 variant of Red Fluorescent Protein, also called RedStar) gene, an IRES, a blasticidin resistance gene (BSD), a polyA signal and a loxP site flanked hygromycin resistance gene cassette. From this allele the blood enhancer element (Rr137) in intron 1 was deleted. The hygro cassette was removed through subsequent cre-mediated recombination. Fluorescence microscopy and FACS analysis confirms the lack of expression from this allele in the hematopoietic system. (J:241018)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hhex Mutation: 15 strains or lines available

Notes

Clones HBD-7.5 and HBD-18.1 were selected for injection into blastocysts to generate the HBD mouse line. It is not specified which of these was, or if both were, used for experiments.

References

• Original: J:241018 Migueles RP, et al., Transcriptional regulation of Hhex in hematopoiesis and hematopoietic stem cell ontogeny. Dev Biol. 2017 Apr 15;424(2):236-245
• All: 1 reference(s)