Idh1^tm1Hyan
Targeted Allele Detail

Summary

• Symbol: Idh1^tm1Hyan
• Name: isocitrate dehydrogenase 1 (NADP+), soluble; targeted mutation 1, Hai Yan
• MGI ID: MGI:5906460
• Synonyms: Idh1^LSL:R132H
• Gene: Idh1 Location: Chr1:65197775-65225638 bp, - strand Genetic Position: Chr1, 32.91 cM
• Alliance: Idh1^tm1Hyan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241022
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/Ola

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The following elements were inserted into intron 2: a loxP site, a stop cassette, an FRT site flanked neomycin resistance gene cassette and a second loxP site. Exon 3 was replaced with an exon with a GA>AC substitution changing codon 132 from an arginine to a histidine codon (p.Arg132His). This creates a conditional-ready knock-out allele that will only express a transcript with the mutated exon 3 after cre recombination. The missense mutatation replicates one commonly found in human gliomas. (J:241022)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Idh1 Mutation: 42 strains or lines available

References

• Original: J:241022 Pirozzi CJ, et al., Mutant IDH1 Disrupts the Mouse Subventricular Zone and Alters Brain Tumor Progression. Mol Cancer Res. 2017 May;15(5):507-520
• All: 3 reference(s)