Six3^tm5Gco
Targeted Allele Detail

Summary

• Symbol: Six3^tm5Gco
• Name: sine oculis-related homeobox 3; targeted mutation 5, Guillermo Oliver
• MGI ID: MGI:5905043
• Synonyms: Six3^neo
• Gene: Six3 Location: Chr17:85921036-85933619 bp, + strand Genetic Position: Chr17, 55.42 cM
• Alliance: Six3^tm5Gco page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240473
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Epitope tag, Hypomorph)
• Mutation: Insertion
• Mutation details: A loxP site flanked neomycin resistance gene cassette was inserted upstream of exon 1. A 15 amino-acid Avi tag was inserted in-frame with the gene so that the resulting translated peptide would have an N-terminal biotinylation tag. The insertion of the neo cassette is intended to interfere with transcription of the gene and was shown by RT-qPCR to indeed reduce expression to around 27% of wild-type. (J:240473)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Six3 Mutation: 13 strains or lines available

References

• Original: J:240473 Geng X, et al., Six3 dosage mediates the pathogenesis of holoprosencephaly. Development. 2016 Dec 01;143(23):4462-4473
• All: 1 reference(s)