Mtfmttm1c(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Mtfmttm1c(KOMP)Wtsi |
Name: |
mitochondrial methionyl-tRNA formyltransferase; targeted mutation 1c, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:5904492 |
Synonyms: |
MtfmtLoxP |
Gene: |
Mtfmt Location: Chr9:65343064-65360336 bp, + strand Genetic Position: Chr9, 35.31 cM, cytoband D
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Alliance: |
Mtfmttm1c(KOMP)Wtsi page
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IMPC: |
Mtfmt gene page |
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Germline Transmission: |
Earliest citation of germline transmission:
J:239776
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Parent Cell Line: |
JM8.F6 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Conditional ready) |
Mutation: |
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Insertion
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Mutation details: The L1L2_gt2 cassette was inserted at position 65347199 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 65347986. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s).
In vivo, a single T to C mutation at position 4 of the central spacer region in the distal (3') loxP site was found, which completely inhibited the recombination reaction in two conditional mouse models. High levels of cre expression in vitro could result in recombination.
(J:239776)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Mtfmt Mutation: |
18 strains or lines available
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Original: |
J:239776 Arguello T, et al., Cre recombinase activity is inhibited in vivo but not ex vivo by a mutation in the asymmetric spacer region of the distal loxP site. Genesis. 2015 Nov;53(11):695-700 |
All: |
2 reference(s) |
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