Jmjd1c^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Jmjd1c^{tm1c(EUCOMM)Wtsi}
• Name: jumonji domain containing 1C; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5904450
• Synonyms: Jmjd1c^f
• Gene: Jmjd1c Location: Chr10:66932189-67092105 bp, + strand Genetic Position: Chr10, 34.85 cM
• Alliance: Jmjd1c^{tm1c(EUCOMM)Wtsi} page
• IMPC: Jmjd1c gene page

Mutation origin

• Mutant Cell Line: EPD0628_4_H11
• Germline Transmission: Earliest citation of germline transmission: J:232419
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 67059451 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 67062822. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:232419)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Jmjd1c Mutation: 222 strains or lines available

References

• Original: J:232419 Zhu N, et al., MLL-AF9- and HOXA9-mediated acute myeloid leukemia stem cell self-renewal requires JMJD1C. J Clin Invest. 2016 Mar 1;126(3):997-1011
• All: 3 reference(s)