Mirc35^tm2Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirc35^tm2Wtsi
• Name: microRNA cluster 35, including Mir182 through Mir183; targeted mutation 2, Wellcome Trust Sanger Institute
• MGI ID: MGI:5898541
• Synonyms: Mir182-Mir183 cluster 6N5 KO, Mir182^{tm1(mirKO)Wtsi}
• Gene: Mirc35 Location: Chr6:30165917-30169736 bp Genetic Position: Chr6, Syntenic

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_35B1, mirKO_ES_PuDtk_35B2, mirKO_ES_PuDtk_35B3, mirKO_ES_PuDtk_35B5, mirKO_ES_PuDtk_35B6, mirKO_ES_PuDtk_35B7, mirKO_ES_PuDtk_35B8, mirKO_ES_PuDtk_35B9, mirKO_ES_PuDtk_35B10, mirKO_ES_PuDtk_35B11, mirKO_ES_PuDtk_35B12, mirKO_ES_PuDtk_35C1, mirKO_ES_PuDtk_35C2, mirKO_ES_PuDtk_35C3, mirKO_ES_PuDtk_35C4, mirKO_ES_PuDtk_35C5, mirKO_ES_PuDtk_35C6, mirKO_ES_PuDtk_35C7, mirKO_ES_PuDtk_35C8, mirKO_ES_PuDtk_35C9, mirKO_ES_PuDtk_35C10
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: The cluster of microRNA genes was replaced via homologous recombination by a LoxP-F3-PGK-EM7-Purotk-bpA-LoxP-FRT targeting vector with 5.5kb (5') and 3.3kb (3') arms of homology. Briefly, this is a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters and flanked by LoxP and F3/FRT sites. The deletion starts at position 30169773 and ends at position 30165900 of Chromosome 6 (Genome Build 38). (J:94077, J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc35 Mutation: 4 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 2 reference(s)