Mirlet7i^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirlet7i^tm1Wtsi
• Name: microRNA let7i; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5898226
• Synonyms: Mirlet7i^{tm1(mirKO)Wtsi}
• Gene: Mirlet7i Location: Chr10:122821545-122821629 bp, - strand Genetic Position: Chr10, 72.1 cM

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_27B12, mirKO_ES_PuDtk_31D1, mirKO_ES_PuDtk_31D4, mirKO_ES_PuDtk_31D6, mirKO_ES_PuDtk_31D8, mirKO_ES_PuDtk_31D9, mirKO_ES_PuDtk_31D10, mirKO_ES_PuDtk_31D11
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion starting at position 122422838 and ending at position 122422692 of Chromosome 10 (Genome Build 38). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and an FRT site. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirlet7i Mutation: 2 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 1 reference(s)