Mirc9^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirc9^tm1Wtsi
• Name: microRNA cluster 9, including Mir99b through Mir125a; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5898224
• Synonyms: Mir99b-Mir125a cluster 17N1 KO, Mir99b^{tm1(mirKO)Wtsi}
• Gene: Mirc9 Location: Chr17:18050450-18051141 bp Genetic Position: Chr17, Syntenic

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_36E1, mirKO_ES_PuDtk_36E3, mirKO_ES_PuDtk_36E4, mirKO_ES_PuDtk_36E5, mirKO_ES_PuDtk_36E6, mirKO_ES_PuDtk_36E7, mirKO_ES_PuDtk_36E8, mirKO_ES_PuDtk_36E10, mirKO_ES_PuDtk_36E11, mirKO_ES_PuDtk_36E12
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The cluster of microRNA genes was replaced via homologous recombination by a LoxP-F3-PGK-EM7-Purotk-bpA-LoxP-FRT targeting vector with 5.5kb (5') and 3.3kb (3') arms of homology. Briefly, this is a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters and flanked by LoxP and F3/FRT sites. The deletion starts at position 17967123 and ends at position 17967541 of Chromosome 17 (Genome Build 38). (J:94077, J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc9 Mutation: 2 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 2 reference(s)