Mirc27^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirc27^tm1Wtsi
• Name: microRNA cluster 27, including Mir497 through Mir195a; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5898123
• Synonyms: Mir497-1/Mir195a cluster 11N1 KO
• Gene: Mirc27 Location: Chr11:70125543-70125961 bp Genetic Position: Chr11, Syntenic

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_30D3, mirKO_ES_PuDtk_30D4, mirKO_ES_PuDtk_30D5, mirKO_ES_PuDtk_30D6, mirKO_ES_PuDtk_30D7, mirKO_ES_PuDtk_30D8, mirKO_ES_PuDtk_30D9, mirKO_ES_PuDtk_30D10, mirKO_ES_PuDtk_30D11, mirKO_ES_PuDtk_30D12
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: The cluster of microRNA genes was replaced via homologous recombination by a LoxP-F3-PGK-EM7-Purotk-bpA-LoxP-FRT targeting vector with 5.5kb (5') and 3.3kb (3') arms of homology. Briefly, this is a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters and flanked by LoxP and F3/FRT sites. The deletion starts at position 70048180 and ends at position 70048680 of Chromosome 11 (Genome Build 38). (J:94077, J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc27 Mutation: 3 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 2 reference(s)