Mir345^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mir345^tm1Wtsi
• Name: microRNA 345; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5898113
• Synonyms: Mir345^{tm1(mirKO)Wtsi}
• Gene: Mir345 Location: Chr12:108802899-108802994 bp, + strand Genetic Position: Chr12, 59.63 cM

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_29B1, mirKO_ES_PuDtk_29B2, mirKO_ES_PuDtk_29B3, mirKO_ES_PuDtk_29B4, mirKO_ES_PuDtk_29B5, mirKO_ES_PuDtk_29B6, mirKO_ES_PuDtk_29B7, mirKO_ES_PuDtk_29B8, mirKO_ES_PuDtk_29B9, mirKO_ES_PuDtk_29B10, mirKO_ES_PuDtk_29B12
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion starting at position 109284774 and ending at position 109284912 of Chromosome 12 (Genome Build 38). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and an FRT site. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir345 Mutation: 1 strain or line available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 1 reference(s)