Mir1949^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mir1949^tm1Wtsi
• Name: microRNA 1949; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5898104
• Synonyms: Mir1949^{tm1(mirKO)Wtsi}
• Gene: Mir1949 Location: Chr18:35687620-35687689 bp, + strand Genetic Position: Chr18, 19.14 cM

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_30G1, mirKO_ES_PuDtk_30G2, mirKO_ES_PuDtk_30G3, mirKO_ES_PuDtk_30G4, mirKO_ES_PuDtk_30G5, mirKO_ES_PuDtk_30G8, mirKO_ES_PuDtk_30G9, mirKO_ES_PuDtk_30G10, mirKO_ES_PuDtk_30G11
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion starting at position 35554529 and ending at position 35554756 of Chromosome 18 (Genome Build 38). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and an FRT site. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir1949 Mutation: 1 strain or line available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 1 reference(s)