Mysm1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mysm1^{tm1c(KOMP)Wtsi}
• Name: myb-like, SWIRM and MPN domains 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5896988
• Synonyms: Mysm1^flox
• Gene: Mysm1 Location: Chr4:94830277-94867337 bp, - strand Genetic Position: Chr4, 43.34 cM
• Alliance: Mysm1^{tm1c(KOMP)Wtsi} page
• IMPC: Mysm1 gene page

Mutation origin

• Mutant Cell Line: EPD0019_1_A05
• Germline Transmission: Earliest citation of germline transmission: J:235706
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_st0
• Mutation details: The L1L2_st0 cassette was inserted at position 94860893 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 94861685. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:235706)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mysm1 Mutation: 44 strains or lines available

References

• Original: J:235706 Belle JI, et al., Repression of p53-target gene Bbc3/PUMA by MYSM1 is essential for the survival of hematopoietic multipotent progenitors and contributes to stem cell maintenance. Cell Death Differ. 2016 May;23(5):759-75
• All: 6 reference(s)