Crb2^tm1.2Wij
Targeted Allele Detail

Summary

• Symbol: Crb2^tm1.2Wij
• Name: crumbs family member 2; targeted mutation 1.2, Jan Wijnholds
• MGI ID: MGI:5896823
• Synonyms: Crumbs2^-
• Gene: Crb2 Location: Chr2:37666261-37689115 bp, + strand Genetic Position: Chr2, 24.19 cM
• Alliance: Crb2^tm1.2Wij page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227336
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site and FRT flanked neomycin selection cassette were inserted in intron 9a and a second loxP site was inserted in exon 13 in the 3' untranslated region. The selection cassette was subsequently removed from the properly targeted allele using flp recombinase, and exons 10 through the coding part of exon 13 were removed via Cre-mediated recombination in the germline. (J:227336)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crb2 Mutation: 53 strains or lines available

References

• Original: J:227336 Ramkumar N, et al., Protein O-Glucosyltransferase 1 (POGLUT1) Promotes Mouse Gastrulation through Modification of the Apical Polarity Protein CRUMBS2. PLoS Genet. 2015 Oct;11(10):e1005551
• All: 1 reference(s)